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Figure 3. Protein–protein interaction network of the <t>CD46</t> proteins and immune-related proteins and tight junction proteins. Fifteen pro- teins were linked into the network. CDH1 = E-cadherin; OCLN = occluding; TJP1 = ZO-1; LTA = TNF-β. Known interactions are represented:
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Figure 3. Protein–protein interaction network of the <t>CD46</t> proteins and immune-related proteins and tight junction proteins. Fifteen pro- teins were linked into the network. CDH1 = E-cadherin; OCLN = occluding; TJP1 = ZO-1; LTA = TNF-β. Known interactions are represented:
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Figure 3. Protein–protein interaction network of the <t>CD46</t> proteins and immune-related proteins and tight junction proteins. Fifteen pro- teins were linked into the network. CDH1 = E-cadherin; OCLN = occluding; TJP1 = ZO-1; LTA = TNF-β. Known interactions are represented:
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Figure 3. Protein–protein interaction network of the <t>CD46</t> proteins and immune-related proteins and tight junction proteins. Fifteen pro- teins were linked into the network. CDH1 = E-cadherin; OCLN = occluding; TJP1 = ZO-1; LTA = TNF-β. Known interactions are represented:
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membrane cofactor protein (cd46)  (Namiki Shoji Co)
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Figure 3. Protein–protein interaction network of the <t>CD46</t> proteins and immune-related proteins and tight junction proteins. Fifteen pro- teins were linked into the network. CDH1 = E-cadherin; OCLN = occluding; TJP1 = ZO-1; LTA = TNF-β. Known interactions are represented:
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soluble forms of membrane cofactor protein (cd46) of complement  (Hasegawa Co Ltd)
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Figure 3. Protein–protein interaction network of the <t>CD46</t> proteins and immune-related proteins and tight junction proteins. Fifteen pro- teins were linked into the network. CDH1 = E-cadherin; OCLN = occluding; TJP1 = ZO-1; LTA = TNF-β. Known interactions are represented:
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Figure 3. Protein–protein interaction network of the <t>CD46</t> proteins and immune-related proteins and tight junction proteins. Fifteen pro- teins were linked into the network. CDH1 = E-cadherin; OCLN = occluding; TJP1 = ZO-1; LTA = TNF-β. Known interactions are represented:
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Figure 3. Protein–protein interaction network of the CD46 proteins and immune-related proteins and tight junction proteins. Fifteen pro- teins were linked into the network. CDH1 = E-cadherin; OCLN = occluding; TJP1 = ZO-1; LTA = TNF-β. Known interactions are represented:

Journal: Journal of dairy science

Article Title: Yak milk-derived exosomes alleviate lipopolysaccharide-induced intestinal inflammation by inhibiting PI3K/AKT/C3 pathway activation.

doi: 10.3168/jds.2021-20175

Figure Lengend Snippet: Figure 3. Protein–protein interaction network of the CD46 proteins and immune-related proteins and tight junction proteins. Fifteen pro- teins were linked into the network. CDH1 = E-cadherin; OCLN = occluding; TJP1 = ZO-1; LTA = TNF-β. Known interactions are represented:

Article Snippet: The membranes were blocked with 5% skim milk-TBS-Tween20 for 4 h at room temperature and incubated overnight at 4°C with the primary antibodies (diluted 1:1,000 in PBS) against proteins of membrane CD46 (cat. # NB500–301, Novus Biologicals Europe; 1:800), HSP-90α (cat. # 8165, Cell Signaling; 1:1,000), CHI3L1 (cat. # LS-B8213, Life Span BioSciences), APOH (cat. # LS-C314171, Life Span BioSciences, 1:300), tight junction protein 1 (ZO1; cat. # 21773–1-AP, Proteintech; 1:1,000), E-cadherin (cat. # 22018–1-AP, Proteintech; 1:1000), AKT (cat. # 10176–2-AP, Proteintech), phosphorylated (P)-AKT (Ser473; cat. # 4060, Cell Signaling), PI3K (cat. # 2524, Cell Signaling), phosphorylated (P)-PI3K (cat. # 4249T, Cell Signaling), NFκ-B (cat. # 10745–1-AP, Proteintech), C3 (cat. # 21337–1-AP, Proteintech), and β-actin (cat. # abs132184, Absin, Absin Bioscience Co. Ltd.; 1:500).

Techniques: